Three Sisters Harvest Edition is a strategic roll-and-write game about backyard farming. It is named after an Indigenous agricultural technique still widely used today: planting three different crops close together. Corn provides a lattice for beans to climb, the beans bring nitrogen from the air into the soil, and the squash provides a natural mulch ground cover to reduce weeds and keep pests away.

Three Sisters Harvest Edition features all the award-winning gameplay of the original edition plus a twelve-chapter campaign mode with new content, gameplay, and components. The campaign is for both group and solo play. The new content can also be used as expansion modules for an individual game of Three Sisters.

Three Sisters Harvest Edition is a fully standalone replacement for Three Sisters.